Background; Fungal infection is a serious problem in Pediatric intensive care unit (PICU) non-neutropenic patients, as risk factors are still poorly defined. The diagnosis remains difficult, and most of previous literatures concerned about fungal infections in neutropenic adults. Objectives; To determine different species, risk factors, underlying illness, diagnosis, treatment, and outcome of fungal infections in PICU patients. Methods; The study included 40 patients out of 197 PICU admissions over 12 months who were found to be at high risk for fungal infections. They were subjected to; Pediatric Risk of Mortality (PRISM III) score and organ failure assessments, Chest X-ray, blood and endo-tracheal aspirate samples (ETA) cultured on Sabouraud’s dextrose agar, fungal species were identified by microscan 9696 semi-automated system, then blood and ETA were examined for fungal antigens (mannan and galactomannan) by Enzyme Linked Immunosorbent Assay test. SPSS test was used for statistical analysis. Results; Fungal infection was diagnosed in 20 patients (50%). Seventeen candida isolates were detected from both ETA and blood, 2 aspergillus isolates from both ETA and blood and 7 yeasts other than candida were isolated from ETA. Significant risk factors found; higher PRISM score, multi-organ system failure, and thrombocytopenia. ETA had a higher diagnostic yield in comparison to blood, and fungal antigens detection was significantly higher than cultures. The overall mortality was 25% in patients at risk for fungal infection, 40% in patient proven to have fungal infection Conclusion; Fungal infection is a serious problem in our PICU, with high mortality and morbidity in spite of treatment. ELISA antigen detection for candida and aspergillus seems to be a useful tool for diagnosis.