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Stromal cell derived factor-1 chemokine gene variant in non- Hodgkin’s lymphomas

Thesis

Last updated: 06 Feb 2023

Subjects

-

Tags

Clinical & Chemical Pathology

Advisors

Farawila, Hala M., Khourshid, Aula M., Fouad, Nivin M.

Authors

El-Agami, Husam-El-Din Abdel-Mughni

Accessioned

2017-07-12 06:42:46

Available

2017-07-12 06:42:46

type

M.Sc. Thesis

Abstract

Chemokines and their receptors regulate the trafficking of immune cells during their development, inflammation, and tissue repair. The single-nucleotide polymorphism (SNP) rs1801157 (previously known as CXCL12-A/ stromal cell-derived factor-1 (SDF1)-3′A) in CXCL12/SDF1 gene was assessed non-Hodgkin's lymphoma (NHL), since the chemokine CXCL12, previously known as SDF1, and its receptor CXCR4 regulate leukocyte trafficking and many essential biological processes, including tumor growth, angiogenesis, and metastasis of different types of tumors. Genotyping was performed by PCR-RFLP (polymerase chain reaction followed by restriction fragment length polymorphism) using a restriction enzyme HpaII cleavage. No significant difference was observed in genotype distribution between NHL patients and healthy controls whereas a significant difference was observed in extranodal affection and treatment outcome between NHL patients and healthy controls. Further studies will be necessary to elucidate the cancer chemokine network.

Issued

1 Jan 2013

DOI

http://dx.doi.org/10.21473/iknito-space/38386

Details

Type

Thesis

Created At

05 Feb 2023