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Selection of potent Probiotic bacterial strain and suitable delivery form to inhibit Mutans streptococci : An in vitro study

Thesis

Last updated: 06 Feb 2023

Subjects

-

Tags

Pediatric Dentistry

Authors

Authman, Shaker Abdel-Aziz

Accessioned

2018-08-26 05:58:33

Available

2018-08-26 05:58:33

type

M.Sc. Thesis

Abstract

Aim: to evaluate the possible inhibitory effect of selected Probiotic bacterial strains against Streptococcus mutans (SM) and to identify the most suitable Dairy product in which most potent Probiotic strain will exhibit inhibitory activity against SM. Material & Methods: Six Probiotic strains including (Lb reuteri ATCC 23272, Lb rhamnosus ATCC7469, Lb acidophilus ATCC 4356, Lb acidophilus TISTR 450, Lb plantarum ATCC 14917 and Bifi bifidium DSM 20082) were tested against SM. Bioyoghurt, stirred fermented milk and kareish cheese were prepared and tested as delivery vehicle for most potent Probiotic strain. Results: All Probiotics in Group 1-6 significantly reduce % survival rate of SM at all ratio subgroups i.e. A- C (ratio of 3:1, 1:1 and 1:3 SM: Probiotic strain, respectively), with exception of Group 6 at ratio subgroup A. With exception of Groups 4 & 5 at ratio subgroup A, statistically significant difference between all Probiotics in the inhibitory activity against SM at all tested ratio subgroups (A-C). Lb reuteri ATCC 23272 displayed strongest inhibitory activity followed by Lb. rhamnosus ATCC7469, Bifi. bifidium DSM 20082, Lb. plantarum ATCC 14917 then Lb. acidophilus ATCC 4356 and last Lb. acidophilus TISTR 450 displayed weakest inhibitory activity. Lb reuteri ATCC 23272 on stirred fermented milk showed strongest inhibitory activity against SM, followed by Bio-yoghurt then kareish cheese, with statistically significant difference between them. Conclusion: Different Probiotics under study reduce the oral carriage of SM with varying degrees. Stirred fermented milk containing Lb reuteri ATCC 23272 is considered the best Probiotic delivery vehicle for dental caries prevention.

Issued

1 Jan 2013

DOI

http://dx.doi.org/10.21473/iknito-space/40411

Details

Type

Thesis

Created At

05 Feb 2023