Aim: To assess the morphometric analysis in azoospermic testicular biopsies.Patients and Methods: One hundred and two azoospermic men were included dividedinto; obstructive azoospermia (OA, n=42) and non-obstructive azoospermia (NOA, n=60).NOA group was subdivided according to testicular sperm extraction (TESE) results into;unsuccessful TESE (n=36) and successful TESE (n=24). The patients were subjected to thefollowing: history taking, general and genital examination, semen analysis, testicularbiopsies for TESE & histopathology and computerized morphometry for testicular biopsies.Results: The mean surface area of seminiferous tubules was significantly increased in OAcompared with NOA testicular biopsies. In NOA testes, the more advanced histologicalpattern demonstrated significant difference compared with the lower one respectively. Themean interstitial tissue area percentage was significantly decreased in OA compared withNOA histopathological patterns except SCO. In NOA testes the interstitial tissue areapercentage was increased in tubular hyalinization > severe hypospermatogenesis > germcell arrest > hypospermatogenesis > SCO pattern. The mean collagen area percentage wassignificantly decreased in OA compared with NOA biopsies. In NOA testes the meancollagen area percentage was increased in SCO pattern > germ cell arrest > tubularhyalinization > severe hypospermatogenesis > hypospermatogenesis.The mean BMthickness was significantly decreased in OA compared with NOA. In NOA testes, the meanBM thickness was increased in SCO pattern > germ cell arrest > tubular hyalinization >hypospermatogenesis > severe hypospermatogenesis. Successful TESE of NOA casescompared with unsuccessful TESE had a significant increase in the mean seminiferoustubular surface area and significant decrease in the mean collagen area percentage and themean basement membrane thickness.Conclusion: The mean surface area is significantly increased in OA compared with NOAbiopsies. The mean collagen area and the mean basement membrane thickness of theseminiferous tubules correlated with advanced spermatogenic activity and successful TESEin NOA cases.