Background: Liver fibrosis results from chronic inflammation of hepatic parenchyma. Progressive accumulation of fibrous tissue eventually leads to cirrhosis and its complications. The severity of liver fibrosis defines the stage of chronic hepatitis and carries with it important clinical implications. Histological scoring systems such as Ishak provide descriptive evaluation of the liver tissue mainly in terms of architectural changes without measuring the amount of fibrosis.Objective: To measure the severity of liver fibrosis quantitatively and to compare this with established methods, such as Ishak scoring system. Materials and methods: Lieca Qwin 500 image analyzer with damaged area morphometry software was used by interactive method to measure the fibrous tissue area based on the different colors of hepatocytes and fibers following staining with Masson’s trichrome stain. 43 cases (38 males, 5 females) recruited into the present study with a mean age of 45.5 years (range 15-58 years). Of these, 40 had chronic viral hepatitis and 3 had chronic non viral hepatitis.Results: Computer morphometry values were highly correlated with results of the Ishak method. The correlation was statistically significant by Chi square (X2) test (P < 0.0001).Conclusion: Quantitative image analysis estimation of liver fibrosis area percentage is simple and accurate method for fibrous tissue evaluation in patients with chronic hepatitis to help in therapeutic approaches.