Purpose: The aim of this study was to evaluate the effect of secondarily splinting implant analogues with resin prior to impression pouring on the passive fit of screw-retained frameworks using single screw test. Materials and methods: The sample of this study consisted of ten patients. All patients were completely edentulous, free of systemic diseases and with adequate interarch distance. The patients received four implants in each arch. Two impression pouring techniques were obtained. Technique 1: master casts generated from non-splinted coping impression technique and the impressions were poured directly without splinting the implant analogues. Technique 2: master casts generated from non-splinted coping impression technique, while implant analogues were splinted with autoploymerized acrylic resin before pouring. Single screw test was used to evaluate the passivity. Results: The result showed greater passivity value for secondary splinting impression pouring technique with statistically significant difference when compared with non-splinting impression pouring technique (P = 0.012). Conclusions: Implant frameworks constructed from secondary splinted technique demonstrate statistical significant passive fit when compared to frameworks constructed from non-splinted technique. Secondary splinting of implant analogues prior to pouring was easy and reliable method to obtain accurate master casts, and non-splinted technique should always be verified to assess accuracy before constructing the framework.