Objectives: The aim of the present work is to evaluate the possible involvement of 647A/C polymorphism of MT-1A gene and low serum zinc level in the susceptibility to type 2 Diabetes Mellitus and its cardiovascular complications.Patients and Methods: This study was conducted on a total number of 58 type 2 diabetic patients and 20 healthy control group. The diabetic group was subdivided into two groups, group 1 consists of 27 type 2 diabetic patients without cardiovascular complications (DNC) and the second group consists of 31 type 2 diabetic patients with cardiovascular complications (DCVD). Patients and controls undergo full clinical examination, laboratory investigations including (fasting and post prandial blood glucose levels, glycated haemoglobin, complete lipid profile and serum zinc). Genomic DNA was extracted from peripheral blood and analyzed for determination of +647 A/C MT1A gene polymorphism using PCR–RFLP.Results: The prevalence of polymorphic genotypes (AC & CC) was statistically significantly increased in diabetic patients than in control group, p=0.01. Also on comparing DCVD with control a high statistical significant increase was observed in AC and CC genotypes compared to AA genotype with P=0.002. No significant difference was found between DCVD and DNC. As regard serum zinc a lower level was observed in DCVD group compared to other group, in addition AC & CC genotypes were associated with significant lower serum zinc level when compared to AA genotype, p= 0.05 and 0.002 respectively.Conclusion: our data support the role of +647 A/C MT1A gene polymorphism in the development of type 2 diabetes and its cardiovascular complications by influencing glycemic control, oxidative stress and zinc level.