Regarding biomedical science, stem cell usage for repair of damaged tissues had captured the imagination of scientists for decades. Our work strategy was to improve the ability of dermal scaffolds for wound healing by their bioactivation using Pancreatic Stem Cells. Stem cell sheets were used to replace 15 mm large full-thickness circular skin wounds. Experiments were classified into 2 groups, one with the PSCs seeded upon the scaffold and the other group used the scaffold alone. This study showed that the combined use of PSCs with the dermal scaffolds has significantly increased the epithelialization and healing capacity in full-thickness wounds forming histological structures similar to epidermis.