Background: Hearing impairment is the most common congenital defect, acquired hearing loss may develop as side effect of using ototoxic drugs. Aim of the work: To assess the effect of vancomycin on hearing of newborns admitted to NICU. methods: this case control study included 100 neonate (50 received vancomycin) and ( 50 didn’t receive vancomycin). History, investigations done during admission& OAE tests were done. Results: OAE tests were normal in both groups indicating vancomycin not ototoxic. conclusion: Using vancomycin is not ototoxic to neaonates when used in standard dose and duration.