Objectives: To evaluate and compare standard sperm parameters, and sperm DNA fragmentation in seminal ejaculates from men whose partners had a history of recurrent pregnancy loss (RPL) and in a control group of men who had recently established their fertility. Methods: Semen samples from 30 patients with a history of recurrent pregnancy loss and 30 men with proven fertility were analyzed according to World Health Organization guidelines using CASA. Sperm DNA fragmentation was detected by the Aniline blue stain assay. Results: When sperm quality of the control group was compared with that of the RPL group, a significant difference was observed in the sperm deformity index and multiple anomaly index, but not in other parameters. The mean number of sperm cells with fragmented DNA was significantly increased in the RPL group (28.77±11.87) compared with control donors (20.3000±8.46). Sperm DNA fragmentation negatively correlates with the sperm velocity parameters measured by CASA. Conclusions: Our data indicate that sperm from men with a history of RPL have a higher incidence of DNA damage and poor sperm velocity parameters than sperm from a control group, and this can explain in part the pregnancy loss in these patients.