In the present study (300) female patients in the child bearing period (age20-45yrs) were selected from those attending the Obstetric andGynecology out patient clinics as well as infertility section in Kasr ElAini hospital. Two hundred females were complaining of vaginaldischarge, itching or both. One hundred were asymptomatic (controlgroup). The females were divided into four groups, Group I included 40pregnant females, Group II included 76 infertile females, Group IIIincluded 84 females using different contraceptive methods, Group IVincluded 100 asymptomatic females. Vaginal swabs were obtained formall female patients for examination by Direct wet mount examination,Giemsa staining method and InPouch TV culture to detect the presence ofTrichomonas vaginalis infection in these studied groups. OSOM test wasdone to randomly selected 100 female patients only. The overallprevalence of infection was (36%). The prevalence of infection in eachgroup was recorded to be 20%, 26%, 52% and 6% in the four groupsrespectively as detected by all tests done collectively. The InPouchculture was found to have the highest sensitivity and specificity (100%),followed by the OSOM test (87.5%) Wet mount had sensitivity of (56%),while Giemsa stain was the least sensitive (49%).