Extended Spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae are multidrug resistant bacteria which can be responsible for nosocomial and community acquired infections. In our study we aim to evaluate the clinical diagnostic performance of the new selective chromogenic medium ChromID ESBL and to evaluate its sensitivity in comparison to double disc synergy (DDST) and combined disc methods. This study was conducted on 50 clinical isolates collected from a variety of samples including 3 pus, 3 sputum and 44 urine samples. ChromID ESBL showed a sensitivity of (100%) while DDST showed a sensitivity of (94%) in relation to combined disc method.