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Effect of phosphodiesterase inhibitors and testosterone on cavernous tissue apoptotic process

Thesis

Last updated: 06 Feb 2023

Subjects

-

Tags

Dermatology, Andrology & STDS

Advisors

Mussttafa, Taymour, Rashed, Layla

Authors

Quttb, Khaled Abdel-Munaem

Accessioned

2017-04-26 11:23:31

Available

2017-04-26 11:23:31

type

M.Sc. Thesis

Abstract

Aim: To assess improved apoptotic process in diabetic rats treated with PDE-5 Is and/or T. Materials and Methods: One hundred and sixty five aged male Sprague Dawley rats (200-250 gm) were divided into; controls, STZ- induced diabetic rats, diabetic rats on sildenafil day after day (2 mg/kg & 4 mg/kg), diabetic rats on 22.5 mg/kg IM T once, diabetic rats on sildenafil and T (2 mg/kg & 4 mg/kg), diabetic rats on 0.45 mg/kg tadalafil day after day, diabetic rats on tadalafil and T, diabetic rats on sildenafil and tadalafil and diabetic rats on sildenafil, tadalafil and T. In dissected cavernous tissues (after 4 weeks) gene expression of iNOS, BAX & Bcl2 was done and MDA, GPx, cGMP were estimated.Results: Cavernous tissues of diabetic rats showed significant decrease in iNOS, cGMP, Bcl2, GPx and significant increase in BAX, MDA compared with the controls. Diabetic rats on sildenafil or tadalafil intake showed significant increase in iNOS, cGMP, Bcl2, GPx and significant decrease in BAX, MDA compared with diabetics. Diabetic rats on T alone showed significant increase in cGMP, Bcl2, GPx and significant decrease in BAX, MDA compared with diabetics. Diabetic rats on sildenafil & T or tadalafil & T showed significant improvement in tested parameters compared with diabetics or diabetic on PDE-5Is alone. Diabetic rats on sildenafil & tadalafil with or without T showed significant improvement of in tested parameters compared with other groups.Conclusion: PDE-5Is and T have an anti-apoptotic cavernous tissue effect separately or combined.

Issued

1 Jan 2009

DOI

http://dx.doi.org/10.21473/iknito-space/31733

Details

Type

Thesis

Created At

05 Feb 2023