This study was designed to evaluate the effect of different surface protections on the marginal adaptation and surface roughness of nano glass-ionomer restorative material. Sixty standardized rounded class V cavities (3mm diameter, 1.5 mm depth and 2mm from the cement-enamel junction) were prepared on the facial and the lingual surfaces of caries free human premolars. Cavities were restored using Ketac N100 nano glass-ionomer restorative material and divided into four main groups according to surface protection used. Group I (control group): no surface protection was used; Group 2: (control group) Vaseline coating surface protection; Group 3: Copal varnish surface protection and Group 4: Ketac Glaze surface protection was used. Each group was further subdivided into three subgroups according to storage period into: 24 hours, three months and six months. Specimens were stored in artificial saliva at 37oC. 120 readings for surface roughness were done after storage of all the specimens. Surface roughness measurements were done using an optical method using USB Digital microscope with a built-in camera connected with a computer to obtain 3D images of surface profile. After storage, restored specimens were sectioned mesio-distal perpendicularly and 20 readings were done for marginal adaptation at tooth restoration interface (area fraction of pores, % of pores) (count, average size and total surface area of pores) using an image analysis technique for each subgroup. Readings were recorded, tabulated and statistically analyzed. Two-way ANOVA revealed statistical significant different between surface roughness values of the two variables (surface protection, aging period) as an independent variable. Using Duncan post-hoc test, Vaseline showed a statistical significant increased values compared to those of the other groups (p≤0.05), while the other surface protection groups showed no significant difference between each other (p≥0.05).