Entamoeba histolytica and Giardia lamblia are very important causes ofdiarrhea due to parasitic diseases. Their prevalence varies in different areas of theworld. In the present study, a trial to evaluate the role of both organisms in cases ofdiarrhea among children and adults was conducted. An enzyme-linkedimmunosorbent assay (ELISA) was used for detection of Entamoeba species andcompared it with microscopic examination. Regarding Entamoeba direct wetexamination was positive in 21.1% of samples, concentration method was positive in22.2% and ELISA was positive in 16.7%. Sensitivity of ELISA was found to be72.2% and specificity was found to be 97.2 %. So, it was found that ELISA is a morespecific test than a sensitive test. Direct examination for Giardia lamblia gavepositive results in 12.4% of samples and 14.4% by concentration method. ELISA testis expensive compared to the conventional microscopic examination, but, it yieldsobjective results and do not require experienced microscopists so can berecommended for survey studies.