Confocal microscopy is an important new imaging technique that offers tremendous promise for the study of in vivo system for corneal changes, general experimental cell biology and medicine.Confocal microscopy provides the ability to section optically, living or in-vitro tissue non- invasively over time. Images are obtained from different depths within the thick specimen. Thus confocal microscopy has made it possible to view biological structure and function at the cellular level under physiological conditions than other methods.Confocal microscopy allows non-invasive in vivo imaging of the ocular surface. Its unique physical properties enable microscopic examination of all layers of the cornea and have been used to investigate numerous corneal diseases: epithelial changes, numerous stromal degenerative or dystrophic diseases, endothelial pathologies, corneal deposits, infections, and traumatic lesions.