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Evaluation of conventional, immunological and molecular methods for the diagnosis of human giardiasis

Thesis

Last updated: 06 Feb 2023

Subjects

-

Tags

Parasitology

Advisors

El-Adawi, Azza E. , Mousa, Hanaa M. , El-Awadhi, Aesha A.

Authors

El-Mallawani, Marwa Adel

Accessioned

2017-07-12 06:40:39

Available

2017-07-12 06:40:39

type

M.D. Thesis

Abstract

The present study was carried out on a total of 85 cases presenting with symptomsthat might be suggestive of intestinal giardiasis. All stool samples were subjectedto: a) microscopical examination using direct wet smear, concentration techniqueand Iron Hematoxylin (Fe-Hx) staining. b) Direct immnnofuorescent assay (DFA).c) DNA extraction followed by a PCR, using nested primers specific for Giardia.Considering the nested PCR (nPCR) as the gold standard test, sensitivity of stoolmicroscopy using the direct examination and concentration technique and that ofstool microscopy using permanent Iron Hematoxylin stain was found to be 66.7%and 72.9% respectively with a statistically significant difference. While thesensitivity of DFA was 85.4% in detection of Giardia infection with a statisticallysignificant difference compared with the nested PCR. No false positive resultswere recorded ; therefore, specificity for all tests used was 100%

Issued

1 Jan 2012

DOI

http://dx.doi.org/10.21473/iknito-space/35691

Details

Type

Thesis

Created At

05 Feb 2023