The aim of the present study was to compare between push out test and shear bond strength test of Endosequence bioceramic root canal sealer to root canal dentin under different preconditioning of root dentin and determine the failure mode on the debonded surfaces by Scanning Electron Microscope (SEM). Materials and methods: Sixty single rooted teeth were mechanically prepared with stainless steel hand endodontic files, using standardized step-back. The prepared root canals were randomly divided into three groups; twenty each; according to different intracanal moisture conditions: Desiccated group (DS): excess distilled water was removed with paper points followed by dehydration with 95% ethanol. Air dried group (D): the canals were dried with low vacuum by using a Luer adapter for 5 seconds followed by 1 paper point for 1 second. Wet group (W): the root canals were flooded with water. Obturations of all canals were done by lateral condensation technique using gutta-percha and Endosequence BC as root canal sealer. Two slices were obtained from each specimen, one middle and the other apical. For the bond strength evaluation, each specimen were subjected to push-out test and shear bond strength test, the maximum force required to cause bond failure were recorded and evaluated under stereomicroscope and scanning electron microscope to determine the failure mode. Result: showed in middle segment, that group (D) showed the statistically significantly highest mean push out bond strength (8.650 MPa). Wet group showed the statistically significantly lowest mean push-out bond strength (3.691 MPa). The result showed in middle segment, desiccated group (DS) showed the statistically significantly highest mean shear bond strength (0.814 MPa). There was no statistically significant difference between group (D) and group (W); both showed the statistically significantly lowest mean shear bond strength values.Conclusion: Bond strength of Endosequence BC differs according to the root canal level whether middle, or apical. Canal final preparation size may influence the bond strength values in different root canal levels.