Eighty four clinical Staphylococcus aureus isolates from 470 patients were examined phenotypically and genotypically for susceptibility to methicillin/oxacillin. Thirty one isolates were methicillin and oxacillin resistant (MRSA) and 53 were methicillin/ oxacillin susceptible. Phenotypically, oxacillin disc diffusion test showed 100% sensitivity and 50% specificity when compared with other phenotypic and genotypic tests. Oxacillin agar screen test, oxacillin E test and microdilution broth test showed 100% sensitivity and specificity. Genotypically, multiplex PCR for the detection of mecA/nucA genes was performed. MecA gene detection by PCR showed concordance with the results of oxacillin agar screen test, oxacillin E test and microdilution broth (100% sensitivity and specificity). The nucA gene was positive with all coagulase positive strains except for one nucA negative. PCR performed on pus specimens directly without culture was found to be 100% positive for mecA and nucA in < 24 hours.One isolate showed resistance by E test to oxacillin, vancomycin, teicoplanin and gentamicin (VRSA??). Genotypicaly, PCR was positive for van B gene, repeatedly. To the best of our knowledge, the present study is the first work to detect VRSA isolate with van B phenotype and PCR confirmed genotype.PCR assays allow faster establishment of effective antibiotic therapy and a reduction of empirical treatments with broad-spectrum antibiotics, which are associated with high costs, toxicity and emergence of resistant pathogens.