Aim: The purpose of this study is to evaluate the horizontal depth of penetration of various endodontic irrigating solutions into dentinal tubules using prepared nanoilver and CHX loaded nanosilver based irrigating solutions compared to other commonly used irrigating solutions as chlorhexidine (2%), sodium hypochlorite (2.5%), and EDTA (17%). Material and methods: twenty five freshly extracted mandibular premolars were included in the study. Teeth were decapitated at cemento-enamel junction and roots of 13 mm length were mechanically prepared. Initial irrigation by NaOCl (2.5%) was used between instruments followed the by EDTA (17%) at the end of the preparation. Samples were randomly distributed into five groups (n=5) according to the irrigating solution used as a final rinse; Group I: Nanosilver + AF dye, Group II: CHX loaded nanosilver + AF dye, Group III: CHX (2%) + AF dye, Group IV: NaOCl (2.5%) and Group V: EDTA (17%) + AF dye. Sample roots were sectioned at 2, 4, 6 and 8 mm from apices. Linear penetration depths of AF dye in each cross section was measured and analyzed in each group by stereomicroscope and Image j software. Results: Results were subjected to analysis using two way ANOVA and Post Tuckey hoc test. Nanosilver group showed the statistically significantly highest mean dye penetration depth both coronally and mid root. CHX loaded nanosilver group showed statistically significantly lower mean value followed by NaOCl group coronally while at mid root CHX loaded nanosilver group and NaOCl group showed no statistically significant difference and lower mean values than nanosilver group. There was no statistically significant difference between the five groups apically.