Objective: This study aimed to evaluate the antimicrobial effect (enterococcus feaclis and staphylococcus aureus and physical properties (flow, setting time, solubility and dimensional changes) of AH plus sealer and Apexit plus sealer alone or mixed with each of Amoxicillin or Clindamycin. Materials and Method: sixty samples of the sealers group were prepared according to manufacturers’ instructions, weighed, and mixed with crushed amoxicillin or clindamycin equilibrated at the analytical balance at concentration 10% of the sealer’s total weight. The samples were classified to two main groups AHplus sealer or Apexit plus sealer each group had thirty samples (n=30).each group divided into three subgroups (each n=10) according to additives to the sealer subgroup I sealer without additives , sub group II sealer mixing with amoxicillin and subgroup III sealer mixing with clindamycin. Each subgroup tested with enterococcus faecalis or staphylococcus aureus by Agar diffusion test. The inhibitory zone was considered to be the shortest distance (mm) from the outer margin of the sealer to the initial point of microbial growth .Results: regardless of antibiotic additives or incubation time, totally it was found that AH plus sealer group recorded higher inhibition zone mean value than Apexit plus sealer group. The difference between both sealer groups was statistically non-significant (p>0.05). Irrespective of sealer groups or incubation time, totally it was found that that amoxicillin modified subgroup recorded the highest inhibition zone mean value followed by clindamycin modified subgroup mean value while the lowest inhibition zone mean value was recorded with control non-modified subgroup. The difference between subgroups was statistically significant (p<0.05). Pair-wise Tukey’s post-hoc showed non-significance (p>0.05) between antibiotics.-modified subgroups. Regardless of antibiotic additives or incubation time, totally it was found that AH plus sealer group recorded higher inhibition zone mean value than Apexit plus sealer group. The difference between both sealer groups was statistically significant (p<0.05).Irrespective of sealer groups or incubation time, totally it was found that that clindamycin modified subgroup recorded the highest inhibition zone mean value followed by amoxicillin modified subgroup mean value while the lowest inhibition zone mean value was recorded with control non-modified subgroup. The difference between subgroups was statistically significant as indicated (p<0.05).Conclusion: The antimicrobial effect against Enterococcus faecalis and Staphylococcus aureus had alteration after addition of antibiotic to sealer.