Introduction: Cardiotoxicity is one of the most serious complications after anthracycline chemotherapy, so early diagnosis, prevention and treatment are crucial .Tissue Doppler imaging (TDI) and 2-D speckle tracking echocardiography (2D-STE) are novel echocardiographic modalities that can be used for early diagnosis of cardiotoxicity.Aim: The aim of this study was to determine the possibility of early detection of anthracyclines induced cardiotoxicity in children with acute hematological malignancies using TDI and 2D-STE.Methods: Thirty pediatric patients with acute hematological malignancies underwent echocardiographic examinations before and after induction chemotherapy. Their median age was 8.5 years ranging from 4 to17 years Conventional echo, TDI, 2D-STE were done. MPI of left and right ventricles, global longitudinal strain, circumferential strain and strain rates, basal, apical rotation and torsion of left ventricle, TAPSE and MAPSE were measured. Results:, After induction chemotherapy; The RV showed both systolic and diastolic dysfunction, as TAPSE , tricuspid E′ & E′/A′ showed significant reduction (p<0.05 , p<0.001, p<0.01 respectively). Moreover the TDI derived RV MPI become significantly prolonged (p<0.01). The LV systolic dysfunction was evident post chemotherapy, as MAPSE, global longitudinal strain, average longitudinal strain rate and basal circumferential strain become significantly reduced (P<0.001, p< 0.01, p<0.05, p=0.05 respectively). However the LV diastolic function and LV MPI didn’t show any significant change after induction chemotherapy. Conclusions: TDI and 2D-STE could be used with other conventional methods for early detection of Anthracycline induced cardiotoxicity. Moreover early after induction chemotherapy the right ventricular cardiotoxicity was much more evident than the left ventricular one.