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Effect of different thawing temperatures on the motility recovery of cryopreserved human sperm

Thesis

Last updated: 06 Feb 2023

Subjects

-

Tags

Dermatology, Venereology & Andrology

Advisors

Fayez, Ashraf H. , El-Ahwani, Tharwat K. , Zaidan, Ashraf S.

Authors

Zaki, Shadi Zaki Saeid

Accessioned

2017-07-12 06:41:13

Available

2017-07-12 06:41:13

type

M.D. Thesis

Abstract

The objective of this work is to investigate the effect of different thawing temperatures on the motility recovery after cryopreservation of the human sperms. The technical aspects of sperm cryopreservation have significantly improved over the last few decades. However, a standard protocol designed to optimize sperm motility recovery after thawing has not yet been established.Fifty semen samples of males complaining of primary infertility for more than one year were selected among those attending a private ART center seeking for ICSI on account of severe oligoasthenoteratozoospermia. Semen samples were thawed at three thawing temperatures: 25 ˚C, 37 ˚C and 40 ˚C. Motility recovery of the spermatozoa in oligoasthenoteratozoosermic cases after cryopreservation was markedly affected negatively at the three thawing temperatures 25 ˚c, 37 ˚c and 40 ˚c. Total motility percentage was better at thawing temperatures 37 ˚c and 40 ˚c than 25 ˚c. On the other hand the rapidly progressive motility percentage was the highest at thwing temperature 40˚c if compared to the other thawing temperatures; 25 ˚c and 37 ˚c. Sperm thawing at 40 ˚C is better to be used to improve motility recovery after sperm cryopreservation.

Issued

1 Jan 2014

DOI

http://dx.doi.org/10.21473/iknito-space/36405

Details

Type

Thesis

Created At

31 Jan 2023