Hyaluronic acid (HA) is a naturally occurring high molecular weight polysaccharide produced mainly by fibroblast and other specialized connective tissue cells in the body. HA is cleared in the liver by very efficient and specific receptor mechanism in sinusoidal endothelial cells. The aim of this study is to assess the relationship between HA level in blood and degree of liver fibrosis and/or cirrhosis in chronic hepatitis C virus infection patients. This study was carried out on 50 patients with various degrees of hepatic fibrosis, 8 chronic HCV patients with minimal fibrosis, 20 chronic HCV patients with mild liver fibrosis, 11 chronic HCV patients with moderate fibrosis, 11 chronic HCV patients with severe fibrosis (cirrhosis), and 20 control healthy subjects. Patients and control group were submitted to thorough history taking, clinical examination, abdominal ultrasonography, liver function tests, and estimation of serum HA level. Liver biopsies were also obtained from patient group for estimating the degree of fibrosis, inflammation and steatosis.The results revealed presence of positive correlation between HA levels and the stage of liver fibrosis. There were significantly high levels of HA in chronic HCV patients with various degrees of liver fibrosis compared to the control group. Also, there were significantly high levels of HA in cirrhotic group compared to other groups of varies degree of hepatic fibrosis. From this study, we conclude that HA is a useful non-invasive marker for estimation of hepatic fibrosis in chronic HCV patients.