The need of serum-free media for the production of monoclonal antibodies by hybridoma cells has been clearly established by many authors (Chen et al., 1993 ; Stoll et al., 1996; Voigt and Zintl, 1999; Legazpi et al., 2005). There are other important problems associated with serum-containing media, the most important being the high protein load, which makes product purification more difficult. Other problems are the undefined composition of serum as well as the production method of fetal serum, which is also ethically questionable. As a consequence, a lot of effort has been devoted in recent years to replace serum in media for the cultivation of mammalian cells (Faint et al., 1990: Hewlett, 1991; Braman and Rajput, 1993).This project aims at large scale production of MAbs through culture and adaptation of hybridoma cells to grow in serum-free media using T-flasks, and spinner -flask other than ascitic fluid induction method. Large scale production of MAbs using this new method could improve productivity, purity and applicability of MAbs.