Male factor is the main or contributing cause in almost fifty percent of infertile couples. Intrauterine insemination is a commonly performed procedure in cases of infertility. It is indicated in couples with male factor infertility.A number of biochemical molecules in spermatozoa have been proposed as potential predictors of male fertility, one of them is platelet activating factor. Endogenous platelet activating factor (PAF) has been proved to have a role in the reproductive physiology. These functions include ovulation, sperm capacitation, acrosome reaction, fertilization, pre-implantation embryo development, implantation and parturtion. In this study, PAF addition during semen preparation was done. After this, the processed semen was used for intrauterine insemination. These steps were performed in couples with male factor infertility and the pregnancy rate was monitored. Platelet activating factor addition was done to enhance the motility and the fertilizing capacity of the spermatozoa and improve the pregnancy rates of intrauterine insemination and controlled ovarian hyperstimulation.