Antiphospholipid syndrome represents about 15% of the causes of recurrent miscarriage, thus it is the most important treatable cause. Aim of the study: To evaluate whether anti annexin V and anti β2-glycoprotein I antibodies can be used as independent risk factors for recurrent spontaneous miscarriage and to investigate whether the determination of anti annexin V and anti β2GPI antibodies combined with that of anticardiloipin antibodies would help to increase the diagnostic sensitivity of antiphospholipid tests. Material and method: 40 patients with history of recurrent miscarriage and 20 healthy fertile females of matching age served as a control group. Anticardiolipin antibodies, anti-annexin V antibodies and anti- β2-glycoprotein I antibodies were measured in both cases and controls by ELISA. Results: Samples positive for aCL IgG and IgM, anti-Anx V IgM and anti-β2GPI IgM antibodies were significantly higher in cases with recurrent miscarriage than in control group: 20/40(50%), 18/40(45%), 17/40(42.5%) and 11/40(30%) respectively with P= 0.000, P=0.001, P=0.005 And P=0.001 respectively. Conclusion: The combined assay off anti-cardiolipin antibodies, anti-annexin V antibodies and anti-beta 2 glycoprotein I antibodies increase the percentage of diagnosed cases of recurrent miscarriage with antiphospholipid syndrome and thus prevent the use of unnecessary anticoagulant therapy. Anti-annexin V antibodies and anti-β2 glycoprotein I antibodies (especially of IgM isotype) can be used as independent risk factors in cases of recurrent miscarriage.