Brain And Acute Leukemia Cytoplasmic (BAALC) overexpression has been reported in acute myeloid leukemia (AML). In the present study, BAALC overexpression was investigated by reverse transcriptase- polymerase chain reaction (RT-PCR) in 30 patients with de novo AML as well as 20 normal subjects as a control group. Internal tandem duplication mutation of Fms – like tyrosine kinase 3 (FLT3/ITD) and length mutation of FLT3 (FLT3/LM) were also analyzed. BAALC overexpression was detected in (46.6%) of the patients while FLT3/ITD was detected in (63.4%) and FLT3/LM (30%). All the control subjects were negative for the three types of mutations. In BAALC positive patients, the mean total leucocytic count is much lower than BAALC negative patients but the difference did not reach a statistically significant level. BAALC positive overexpression was significantly associated with higher blast percentage in peripheral blood and bone marrow blasts. BAALC overexpression was seen in FAB subtypes M1, M2, M4 and M5 and was absent in M3. Most of BAALC positive patients (57%) had normal karyotyping and 56% coexpress FLT3/ITD. The coexpression of both genes showed the worst prognosis, as none of the patients achieved complete remission and 62% of the patients died. Thus, it can be stated that BAALC alone or in conjunction with FLT3/ITD show the worse response to induction chemotherapy.