Objective: To assess the multifrequency tympanometry (MFT) in Menière’sdisease (MD). Methods: MFT was performed in 20 adult patients having definitive MD,and 20 well-matched controls. Measurements included: Tympanograms for admittance (Y),conductance (G), susceptance (B), and phase angle (∅) at different probe frequencies andresonant frequency (RF). Y, B, and G tympanograms were done at R.F and at 2000 Hz.Amplitude, tympanometric width (TW), peak pressure value, and +200 daPa value of G at2000 Hz were determined. Results: The RF of affected ears of MD (group IA) (607.50 Hz± 217.20) is lower than that of the non-affected ears MD (group IB) (642.50 Hz ± 216.60),which in turn is lower than the controls’ ears (group II) RF (725.00 Hz ± 219.15) however,this difference was not statistically significant (p < 0.05). There was a statistically significantdifference among the 3 groups as regards +200 daPa value There was no statisticallysignificant difference among the 3 groups as regards the Δ ∅, or the amplitude or peakpressure value of G at 2000 Hz. The mean TW of group IA was 155.5 daPa ± 104.69 and ishigher than that of group IB (132.25 daPa ± 80.91). Both groups show higher means than thatof group II (88 daPa ± 31.89). This difference was statistically significant. This suggests thatMD results in modification of G tympanograms at 2 KHz, and there may also be sub-clinicalaffection of the asymptomatic ears of MD. There was no correlation between quantitativetympanogram criteria and duration of MD or pure tone thresholds except for a statisticallysignificant direct correlation between RF and age and hearing level at 250, 1000 and 4000 Hz.Conclusion: MFT specially the measurement of RF of the middle ear and measurementof the TW of G tympanogram at 2 kHz has a role in the assessment of MD and its possiblesub-clinical bilaterality.