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Histological and immunohistochemical study of some neuroendocrine cells in gastric mucosa of albino rat in different ages

Thesis

Last updated: 06 Feb 2023

Subjects

-

Tags

Histology

Advisors

Filubbus, Suhair A., Murquss, Mary A., Abou-El-Nour, Rahma K.

Authors

Abdel-Hakim, Khaled Ragab

Accessioned

2017-07-12 06:42:31

Available

2017-07-12 06:42:31

type

M.Sc. Thesis

Abstract

The aim of this study was to localize the histological and immunohistochemical distribution of various enteroendocrine cells in the mucosa of the stomach of rats of various ages (weaning, adults, old) and try to find an explanation for their role in normal and disease conditions.All rats were anaesthetized with thiopental sodium, sacrificed and the stomachs were dissected out. The fundus was taken opened from the greater curvature while the pylorus was taken few millimeters proximal to the pyloric sphincter. The specimens were treated in a proper way to prepare 5µm thick paraffin sections which were stained by H&E ,silver (Grimelius method) for argentaffin cells and immunohistochemical techniques localizing chromogranin A, gastrin and somatostatin positive cells. Data of area percentage of each of the immune reaction was measured, tabulated and statistically analysed.Result: Enterondocrine cells were most abundant in gastric mucosa of weaning rats, less in adult rats and least in old age in both fundic and pyloric regions.Conclucion: It has been observed that all neuroendocrine were significantly increased in the fundus and pylorus at the age of weaning as compared to adult and old groups.

Issued

1 Jan 2012

DOI

http://dx.doi.org/10.21473/iknito-space/38043

Details

Type

Thesis

Created At

31 Jan 2023