Objective: To evaluate the reliability of vaginal fluid urea and creatinine for the diagnosis of premature rupture of membranes. Materials and Methods: 150 pregnant women were recruited. Group I: 50 patients with confirmed PROM, group II: 50 patients with suspected PROM, group III: 50 normal pregnant women. All patients underwent ultrasonography, speculum examination for amniotic fluid pooling, nitrazine paper and vaginal washing fluid urea and creatinine sampling. Results: The mean vaginal fluid urea levels in group I, II, and III were 13.23 +/- 5.69, 6.44 +/- 4.11 and 2.29 +/- 3.12 mg/dl, respectively, where the difference was statistically significant (P < 0.001). The sensitivity, specificity, positive predictivity, and negative predictivity were 80%, 100%, 100% and 83.3% in detecting PROM by evaluation of vaginal fluid urea with a cut-off value of >6.5 mg/dl. The mean vaginal fluid creatinine concentrations of group I, II, and III were 0.96 +/- 0.55, 0.46 +/- 0.33 and 0.06 +/- 0.13 mg/dl (P < 0.01). The sensitivity, specificity, positive predictivity, and negative predictivity were 84%, 100%, 100% and 86.2% in detecting PROM by evaluation of vaginal fluid creatinine concentration with a cut-off value of >0.5 mg/dl. Conclusion: Vaginal washing fluid urea and creatinine determination for the diagnosis of PROM is a reliable, simple and rapid test.