The ocular surface describes the entire epithelial surface of the external eye including the corneal, conjunctival and limbal epithelia as well as the preocular tear film. The stem cells are the provider for the regeneration of the corneal epithelium. The management of ocular surface disorders using limbal stem cell transplantation has benefited from major breakthroughs in recent years. Understanding the role of limbal stem cells have led to great improvements in both visual acuity and quality of life for these patients through different surgical techniques.