Soft-tissue or muscle hemorrhages are seen in a minority of hanging cases. The aim of the current study is to investigate the effectiveness blood markers, macroscopic, microscopic examination, using routine histological stains and immunohistochemical study using Anti- Myoglobin. This was performed on 100 autopsied cases at the different morgues of the Ministry of Justice - Egypt. Positive findings included opaque fibers observed in almost all muscle specimens (92%), and were seen only at the region of the just beneath the compression mark on the neck, irrespective of material used in suspension. It is concluded that myoglobin immunostaining study is an important supplementary evidence of compression of the neck, especially when the compression mark on the neck is indefinite macroscopically.