Glaucoma is the leading cause of irreversible blindness in the world. Identification of the individuals with glaucoma before they develop symptomatic visual loss is critically important to prevent or slow the progression of damage. Optical coherence tomography (OCT) is one promising technology that has been developed to assess tissue thickness in vivo, such as that of the retinal nerve fiber layer. OCT permits high resolution cross-sectional imaging of biological tissue using light. Heidelberg retina tomograph (HRT) is a confocal scaning laser ophthalmoscope that enable one to receive three - dimensional images of the optic nerve head. In our work we assessed the optic disc and the retinal nerve fiber layer in normal, ocular hypertensive (OHT) and glaucomatous eyes with HRT and OCT.