The emergence and rapid dissemination of carbapenemase-producing enterobacteriaceae (CPE) worldwide is a cause for concern. Fecal carriage with carbapenemase-producing enterobacteriaceae (CPE) remains scarcely investigated except for selected patients at risk, mostly during outbreaks. Outbreaks of CPE, primarily Klebsiella pneumoniae, have been reported recently in several regions worldwide. The aim of this work is to evaluate the prevalence of intestinal colonization with CPE in non hospitalized patients and to assess a microbiology protocol for screening these isolates in fecal material. Our study started with 600 fecal samples 450 from non hospitalized patients (group I) and 150 from hospitalized patients (group II) and all of them were screened for CPE. From these fecal samples, 12 were selected for genotypic analysis using convential PCR, 2 of them were non hospitalized patients (group I) and 10 were hospitalized patients (group II) based on positive Chrom agar-KPC, reduced susceptibility to one or more of carbapenems by disk diffusion method, and/or positive Modified Hodge Test.