Neonatal sepsis is still one of the major health problems all over the world. This is a retrospective study to detect the incidence, risk factors, common clinical signs, causative organisms and the outcome of neonatal sepsis among neonates admitted to the NICU of Pediatric Hospital of Cairo University. The study included 136 neonates who were admitted to the NICU of Pediatric hospital of Cairo University during the year 2011 and diagnosed as having neonatal sepsis both on clinical basis and laboratory data. Klebsiella was found to be the commonest causative organism isolated (17.5%), followed by CONS (9.5%) and MRSA (4.8%). The outcome of neonatal sepsis showed that 66.2% of cases were discharged home while 33.8% of cases died. Prematurity and respiratory distress (RD) were significantly associated with higher risk of mortality among neonates with sepsis.