The aim of this study was to establish whether exercise following radiopharmaceutical administration [Tc-99m MDP] has a real impact on the quality of skeletal scintigraphy. A one hundred patients referred for bone scanning for various reasons over a six months period were issued with instructions to do bone scan then do exercise and repeat the image. Computer based equal regions of interest (ROI) were done; over the mid- femoral diaphysis [bone ROI] and the adjacent soft tissue area [soft tissue ROI]. A bone to soft tissue ratio was obtained for each case [B: ST]. Statistically significant difference was found between the ratios’ means. Hence, we concluded that exercise following Tc-99m MDP injection has significant effect on the bone: soft tissue ratio and in turn, the skeletal scintigraphic quality.