Objective: Screening a group of adolescent (10-19 years) Egyptians with microcytic hypochromic anemia for beta- thalassemia trait. Study Design and Setting: Cross-sectional descriptive study conducted on 200 adolescent Egyptians with microcytic hypochromic anemia with mean corpuscular volume (MCV) value < 78 fL provided there is no family history of hemoglobinopathy. All patients attended the Hematology Laboratory Clinic at Kasr AlAiny Manial Hospital in the period from early February till the end of April, 2013. Their blood samples were investigated for: Serum Fe, TIBC, HbA, HbA2 and HbF levels. The geographic accessibility and the availability of the patients in Kasr Alainy Hospital served the limited time plan allowed for the research to be conducted in. Conclusion: Screening for beta-thalassemia trait among the study group revealed the presence of 10 carriers (5%). A blood picture presenting with low mean corpuscular volume (MCV) of <78fL and low mean corpuscular hemoglobin (MCH) of <27pg provides a useful screening tool for beta-thalassemia trait. HbA2 is essential in diagnosing beta-thalassemia trait. Serum iron (Fe) measurement is not essential in diagnosing beta-thalassemia trait. Its importance is in excluding concomitant Fe-deficiency anemia.