Capacity building of nursing students to develop appropriate knowledge and skills in infection control (IC) is crucial for health care quality. Nurses do receive IC training before graduation, however; their actual IC performance is still unfavorable. Therefore, there is a need to provide evidence -based strategies in training practices for nursing students. Objectives: To design and conduct training strategy based on needs detected by the baseline assessment, and evaluates its impact on knowledge and skills of nursing students. Methodology: An operations research - interventional study- pretest - posttest design, it was conducted at the Technical Institute of Nursing, Cairo University (TIN-CU).Fifty eight students participated in the study(pretest-training-immediate posttest) however 38 students continued to participate in the maintenance posttest. Results: TIN-CU IC curriculum was assessed according to the basic national guidelines for IC(10 chapters) by Ministry of Health and Population(MOHP), it was found that 7 chapters of the national guidelines were present in the institute’s original curriculum though some important items were missing and 3 chapters were completely absent from the institute’s original curriculum . Regarding the total knowledge and skills scores of the trainees significant improvement was achieved immediately after the training (p value<0.001), significant decline was found between immediate and maintenance posttest (p value<0.001). Regarding the total knowledge score of the trainees, their maintenance posttest level was significantly higher than pretest level (p value<0.001), however for the total skills score no significant change was found (p value=0.772). Conclusion: Introducing IC training to nursing students led to immediate improvement of their knowledge and skills, this improvement was sustained regarding the IC knowledge and was lost regarding the IC skills. Recommendations: Upgrading IC curriculum of nursing students and conducting further research about IC teaching and training by different approaches.