Background: Chronic periodontitis is the most frequently occurring form of periodontitis resulting in inflammation within the teeth supporting tissues, progressive attachment loss and bone loss .Interleukin-17 (IL-17) is a T-cell-derived cytokine that may play an important role in the initiation or maintenance of the pro-inflammatory response and has recently been found to stimulate osteoclastic resorption. Aim of the study: The purpose of the present study is to determine the level of IL-17 in samples of Gingival Crevicular Fluid (GCF) from chronic periodontitis patients and to compare it with healthy controls. Method: GCF samples were collected from 20 patients from periodontally affected sites (probing depth ≥5 mm, attachment loss ≥3 mm). The comparison with healthy individuals was carried out by collecting GCF samples from20 healthy volunteers. GCF was collected using a paper strip and ELISA was performed to determine the total amount of IL-17. Results: The mean concentration of IL-17 was significantly higher in group 2 when compared with group 1. Gingival index Plaque index and Pocket depth scores were significantly higher in group 2 when compared with group1. Conclusions: The total amount of cytokine IL-17 in GCF samples is significantly increased in periodontal disease.