Background: Helicobacter pylori (Hp) is a gram-negative bacillus responsible for one of the most common infections found in humans worldwide .It is present in about 50% of the global population . It was found that it is closely associated with antral gastritis, gastric and duodenal ulcers in adults and children. H pylori was classified by the world health organization as a class I carcinogen for gastric cancer and currently this organism is considered the most common etiological agent of infection related cancers representing 5.5% of global cancer burden. Aim : To compare between sequential therapy and standard triple therapy for treatment of Helicobacter pylori.Patients and Methods: This is a prospective study carried out on 41 patients selected from dyspeptic patients attending Ahmed Maher teaching Hospital from October 2011 till November 2012, all patients were Helicobacter pylori infected as evidenced by endoscopic biopsy and Histopathological examination . 20 patients were randomly assigned to sequential therapy (20 mg PPI twice daily, amoxicillin1g twice daily for the first 5 days and 20mg PPI twice daily ,clarithromycin 500mg twice daily and tinidazole 500mg twice daily for the second 5 days, While 21 patients were randomly assigned to standard triple therapy (20mg PPI twice daily , amoxicillin 1g twice daily,clarithromycin500mg twice daily for 10 days) response to therapy was evaluated by Helicobacter Pylori stool antigen 45 days after cessation of therapy.Results:eradication rates of 10 day-sequential therapy and PPI-based triple therapy were 6 (30%) and 7(33.3%) respectively, (p= 0.5)which showed no statistically significant difference between the 2 studied groups.Conclusion : The 10 day-sequential therapy regimen failed to achieve significantly higher eradication rates than PPI-based triple therapy.