Beta
41367

Role of sildenafil as a factor modulating skeletal muscle contractility in cases of induced diabetic rats

Thesis

Last updated: 06 Feb 2023

Subjects

-

Tags

Physiology

Advisors

Khuwayled, Akef H. , Abdel-Wahhab, Zainab , Shaker, Ulfat G.

Authors

Salem, Nour-El-Din Beshir

Accessioned

2017-07-12 06:40:19

Available

2017-07-12 06:40:19

type

M.Sc. Thesis

Abstract

Aim of work: This study was designed to evaluate the effect of sildenafil citrate on skeletal muscle contractility in type 2 diabetic rats. Materials and methods: Diabetes was induced by feeding rats with a high fat diet (HFD) for 2 weeks followed by an intraperitoneal injection of streptozotocin (35 mg/kg body weight). The considered rats were divided into four groups containing ten animals for each, group1: normal control, group 2: diabetic group, group 3: diabetic treated with 5mg/kg/day sildenafil for 4 weeks and group 4: diabetic treated with 10mg/kg/day sildenafil for 4 weeks. By the end of the experimental period rats were sacrificed, blood samples were taken and serum was isolated for estimation of glucose, insulin, TNF-α, IL-1β, IL-6 and lipid profile parameters. In addition, diaphragm contractility was determined in the studied groups. Results: In the diabetic group, levels of glucose, TNF-α, IL-1β, IL-6, glycosylated hemoglobin, HOMA-IR, total cholesterol, triglycerides, LDL-cholesterol and vLDL-cholesterol were significantly increased, while contractility index, serum HDL-cholesterol and insulin levels were decreased. Both doses of sildenafil significantly reversed these alterations. Moreover, supplementation with either dose significantly ameliorated the diminished diaphragm contractility. Conclusion: The current study showed that sildenafil citrate has pronounced useful effects on skeletal muscle contractility via ameliorating the deteriorated metabolic parameters in type 2 diabetic rats.

Issued

1 Jan 2012

DOI

http://dx.doi.org/10.21473/iknito-space/35304

Details

Type

Thesis

Created At

31 Jan 2023