Intratubular germ cell neoplasia (ITGCN), is a pre-invasive precursor of testicular germ cell tumors, an increased risk of harbouring (ITGCN) has been reported in men with a history of cryptorchidism, in contralateral testes of men previously treated for unilateral germ cell tumor, in intersex patients, in men with extragonadal germ cell tumor and in infertile men so a testicular biopsy is necessary to diagnose (ITGCN) . Aim of the work: The aim of this retrospective study is to find the prevalence of ITGCN among testicular biopsies from azoospermic patients attending Kasr EL-Ainy Hospital between the years 2005 – 2007. MATERIAL AND METHODS: This retrospective study was done from patients underwent testicular biopsies on Kasr El-Ainy hospital for period (2005 - 2007), for each patient we recorded the age, duration of infertility, FSH level, fresh mount and pathology, every slide was examined under light microscopy 40x magnification for histological evaluation and the suspected cases of (ITGCN) according to WHO was recorded and there paraffin sections were treated with placental alkaline phosphatase (PLAP) stain, a classic immunohistochemical marker of ITGCN for evaluation. RESULTS: Among 415 patients, (ITGCN) examination was confirmed in 4 patients which is about (1%) of cases. CONCLUSION: for all patients at high risk of (ITGCN), PLAP stain should be routinely done to the testicular biopsy, this would allow earlier detection and proper management of such serious life threatening condition and all azoospermic patients undergoing TESE should be examined histopathologically and suspicious cases of CIS (ITGCN) should be evaluated by PLAP stain.