Premature rupture of membranes (PROM) is the rupture of fetal membranes at least 24h before onset of labour ,while preterm premature rupture of membranes ( PPROM) is the rupture of fetal membranes which proceed the onset of labour for more than 24hours before 37 weeks of gestation. Correct diagnosis of PROM has great importance because failure of diagnosis can lead to unwanted obstetric complications. The false diagnosis of membrane rupture can lead to inappropriate interventions such as hospitalization or induction of labor. Therefore, many diagnostic test has been discovered including many biochemical markers as B-HCG, urea , creatinine , thyroid hormones fetal fibronectin and ILGF-1 but non of them proved to be the golden standard in diagnosis of (PROM). Any biochemical test used to establish a correct diagnosis must be reliable, simple and rapid. PAMG-1 detection in cervicovaginal discharge is a new diagnostic test for ROM .it can be used as a marker for diagnosis due to its unique characteristics (i.e., high concentration in the amniotic fluid, low level in blood , and extremely low background level in cervicovaginal secretions with intact fetal membranes). Amnisure is the test used for detection of PAMG-1 in cervicovaginal discharge. In this study the result of Amnisure test was compared to other diagnostic test as Ferning, Nitrazine ,pooling and AFI. This prospective case-control study was carried out at Cairo University Hospital. The study included 60 pregnant women between 20-37 weeks of gestation were subdivided into two groups of women. These result proved that the use of Amnisure test is better than other traditionally available technique for diagnosis of PROM. So its our recommendation is to use Amnisure test for diagnosis of PROM, although the cost of the test is high in comparison to other diagnostic test but the high accuracy and reliability of it is a great benefit.