The antigens used was crude worm antigens ELISA revealed that the sensitivity of S. haematobium and S. mansoni positive subjects was 78.57% for S. mansoni and 87.14% for S. haematobium The soluble adult worm antigens preparation was separated by gel electrophoresis to generate a reference gel used for western blotting. By software image analysis we found that there was a high degree of similarity in band pattern reveled from separation of S. mansoni and S. haematobium adult worm antigens.