Type 2 diabetes mellitus one of the most common syndromes, it results from an inadequate adaptation of the functional pancreatic beta cell mass in the face of insulin resistance. Apoptotic mechanisms could explain insulin deficiency through a reduction in absolute beta cell mass.Apoptosis is programmed cell death or cell suicide, it is an energy-requiring process that involves de novo protein synthesis. In this study, we analyzed serum levels of Fas, FasL, and Bcl2 of 59 subjects recruited from inpatients and outpatients department of Kasr- El Aini hospital and were classified into three groups:Group A: 10 patients with type II diabetes mellitus without microvascular diabetic complications. Group B: 39 patients with type II diabetes mellitus with diabetic microvascular complications (peripheral neuropathy, diabetic retinopathy, diabetic nephropathy).Group C: 10 normal control persons. All patients and control were subjected to full history, Clinical examination and routine laboratory investigations including: Blood picture, urine and stool analysis, fasting and 2 hours post prandial blood sugar, cholesterol and triglycerides estimation, urea, creatinine and 24 hours urinary albumin, ECG and assay of apoptosis markers: Quantitative detection of Fas, FasL, and BcL2. In our study, we found that there’s a high level a of pro apoptotic markers ( Fas, FasL, and Bcl2) in total diabetic subjects than control subjects, and it’s higher in diabetics without microvascular complications than control group (P =<0.05). Also we found that serum levels of (Fas, FasL, and bcl2) of diabetics with microvascular complications are higher than diabetics without microvascular complications. There was significant correlation between FBS and Fas, FasL, and Bcl2 (P value =<0.01). We concluded that hyperglycemia in type 2 diabetes may induce apoptosis in beta cells and this indicated by elevated serum levels of Fas, FasL, and Bcl2 proteins and this apoptotic markers increased in cases of microvascular affection of diabetes (neuropathy, nephropathy and retinopathy) which suggest a role of apoptosis in the pathophysiology of these complications.