Pityriasis rosea (PR) is a common papulosquamous skin disease in which infective agent may be implicated. Reactivation of human herpes virus 7 (HHV-7) and, in some cases human herpes virus 6 (HHV-6) was suggested to occur in PR. The aim of this study is to detect the possibility of HHV 6,7 involvement in the pathogenesis of PR and it's relation with TLRs 2,4. In our study 25 PR patients, and 25 healthy controls were recruited, a skin biopsy from PR lesions was obtained and real-time PCR was performed to measure HHV6,7 loads as well as TLRs 2,4 levels . Our results indicated a significant elevation in TLR2,4 in our cases as well as a significant elevation HHV6,7 loads .Furthermore, we detected a significant elevation TLR 2,4 in HHV7 positive cases. Our results concluded that TLR 2,4 are involved in viral diseases particularly HHVs, and supported the possibility that HHV7 and to a lesser extent 6 are involved in the pathogenesis of PR.