The aim of this work was to investigate the effect of different physiological factors on capillary vasomotion and skin blood flow in the palmer surface of the thumb and the planter surface of the foot of healthy volunteers. Forty-seven volunteers were divided into five groups according to different physiological factors like the age, the gender, the hemoglobin level as well as the smoking habit. Assessment of the Mean and the Maximum blood flow at different skin temperatures (30°C, 35°C, 40°C and 44°C) as well as the % change in blood flow after reactive hyperemia at 30°C were done for each group. Also the frequency and the power of vasomotion at different skin probe temperatures (30°C, 35°C, 40°C and 44°C) were assessed using the Laser Doppler perfusion monitor. Significant effects of the physiological factors were found on these measured parameters. Pearson correlation revealed also a relation between the body surface area and weight and these measured parameters.