NO helps to prevent atherosclerosis. The present study was designed to study the effect of some modulators of L-arginine/NO pathway in the animal model of hypercholesterolemia and atherosclerosis. It was found that dietary supplementation with L-arginine (2% or 4%) improved the endothelium-dependent vasodilatation and regression of atherosclerosis. L-NMMA produced progression of the atherosclerosis and impaired endothelium dependent vasodilatation. Also, fluvastatin improved endothelium-dependent vasodilatation