Background: AML is a heterogeneous disease in clinical character and molecular genetics, we can detect many kinds of abnormal expression of genes. Among them, meningioma 1 (MN1) high expression and phosphatase and tensin homolog (PTEN) low expression are thought to be correlative with incidence of leukemia. Aim of the study: The aim of this study is to determine the expression and prognostic impact of MN1 and PTEN mRNA in AML cells by Real time Reverse Transcription-PCR and to correlate the their expression with other clinical and laboratory prognostic factors. Subjects and Methods: This study was conducted on fifty patients with de novo Acute Myeloid Leukemia from the cases presenting to the clinical pathology department at the National Cancer Institute, Cairo University. In addition to 10 sex and age comparable subjects who were taken as a control group. MN1 and PTEN mRNA was detected by Real time Reverse Transcription-Polymerase Chain Reaction (PCR).Results: MN1 expression was significantly higher (P value <0.001) and PTEN expression was significantly lower (P value =0.002) among the patients compared to the controls, no other statistically significant relation could be established. Conclusion: Although our study suggests a role for MN1 gene and PTEN genes in adult AML, we could not recommend their use as routine diagnostic and prognostic markers for adult AML without further, wider scale, multivariable more comprehensive studies to determine the actual benefit of these markers and their cost effectiveness for the Egyptian population, considering the available resources.